src kinase inhibitor (src inhibitor-1, src i-1  (Millipore)

Journal: PLoS ONE

Article Title: IgE by Itself Affects Mature Rat Mast Cell Preformed and De Novo -Synthesized Mediator Release and Amplifies Mast Cell Migratory Response

doi: 10.1371/journal.pone.0079286

Figure Lengend Snippet: Inhibition of IgE-induced mast cell histamine release, cysLT generation and TNF synthesis by various inhibitors.

Article Snippet: NaCl, KCl, MgCl 2 , CaCl 2 , 2-hydroxyethylpiperazine-N'-ethanesulphonic acid (HEPES), NaOH, glucose, HCl, o -phthaldehyde (OPT), Percoll, hematoxylin, toluidine blue, trypan blue, bovine serum albumin (BSA), PLC/PLA 2 inhibitor (U73122), PI3K inhibitor (LY294002), Src kinase inhibitor (Src Inhibitor-1, Src I-1), RIPA buffer and laminin from human placenta were obtained from Sigma-Aldrich (St. Louis, MO, USA).

Techniques: Inhibition

Journal: PLoS ONE

Article Title: IgE by Itself Affects Mature Rat Mast Cell Preformed and De Novo -Synthesized Mediator Release and Amplifies Mast Cell Migratory Response

doi: 10.1371/journal.pone.0079286

Figure Lengend Snippet: Mast cells were preincubated with U73122 (0.1 µM), Src I-1 (0.01 µM), LY294002 (50 µM) or medium alone for 1 h (none). Then, mast cells were treated with IgE at 5 µg/mL, washed and spontaneous (A), CCL5-induced (B) and TNF-induced (C) migration was examined in a Boyden microchamber. Bars for the positive controls demonstrate the same data set as in . Results are presented as the mean ± SEM of four independent experiments and each experiment was carried out in duplicate (n = 4). *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: NaCl, KCl, MgCl 2 , CaCl 2 , 2-hydroxyethylpiperazine-N'-ethanesulphonic acid (HEPES), NaOH, glucose, HCl, o -phthaldehyde (OPT), Percoll, hematoxylin, toluidine blue, trypan blue, bovine serum albumin (BSA), PLC/PLA 2 inhibitor (U73122), PI3K inhibitor (LY294002), Src kinase inhibitor (Src Inhibitor-1, Src I-1), RIPA buffer and laminin from human placenta were obtained from Sigma-Aldrich (St. Louis, MO, USA).

Techniques: Migration